Modular spectrofluorimeter Fluorolog-3 (Horiba Jobin Yvon)

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Description of the instrument:

The device is designed to produce emission spectra, excitation spectra, measurements of the lifetimes of excited states, to study the molecular dynamics in solution. Double monochromators in the excitation and emission channels, heavy duty xenon lamp as the light source and the detection system operates on the basis of accounts of individual photons, can achieve the unique sensitivity and signal/noise ratio. Device equipped with fluorescence lifetime measurement based on time-correlated single photon counting (TCSPC). It can measure luminescence decay from 0.1 ns to a few seconds. Device also includes an integrating sphere Quanta-φ with fiber optics which enables direct measurement of the quantum yield of luminescence. The device allows obtaining emission spectra with the ability to both radial and frontal observation of the sample.

Excitation sources

  • Xenon lamp with a 450 W ozone protection
  • Xenon flash lamp 150 W

Monochromators

1. Excitation monochromator

- double monochromator by the Czerny-Turner with a plane diffraction grating with an angle of light optimized for ultraviolet (330 nm);
- focusing stored for all wavelengths in the range 200-950 nm;
- adjustable bandwidth from the computer from 0 to 30 nm;
- wavelength accuracy - 0.5 nm;
- maximum scanning speed - 150 nm/s;
- range of the scanning step - 0.0625-100 nm.

2. Emission monochromator - double monochromator by the Czerny-Turner with flat grating with optimized angle of light in the visible region (500 nm);

- focusing stored for all wavelengths in the range 200-950 nm;
- adjustable bandwidth with computer from 0 to 30 nm;
- wavelength accuracy - 0.5 nm;
- maximum scanning speed - 150 nm/s;
- range of the scanning step - 0.0625-100 nm.

3. Spectrograph with a relative aperture of f/4.1, kinematic turret with 3 grating, which allows using 2 channels of registration, with diffraction gratings in the holder 68x68 mm (3 pieces), for the spectral range from 300 nm to 3000 nm.

Detectors

The aspect ratio signal/noise ratio of 5000:1 for Raman band of water

1. Photomultiplier, sensitive in the range of 200-850 nm.

2. Cooled with liquid nitrogen detector for the spectral range 300 - 1700 nm, which is connected to the spectrograph.

3. PbS detector in housing with thermoelectric cooling for the spectral range 1000-3000 nm, which is connected to the spectrograph.

Accessories and add-ons

1. 1. Thermostatic chamber for cuvette (1x1cm) ranging from -20°C to 80°C, with integrated magnetic stirrer.

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2. Sample chamber to operate at liquid nitrogen temperature.

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3. Holder for solid samples

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4. Integrating sphere with 4 inches diameter to determine the quantum yield of the samples.

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 Information on the manufacturer's website 

 

5. Н5. A set of light emitting diodes with wavelengths of 266 nm, 340 nm, 370 nm, 390 nm, 450 nm, 495 nm, 540 nm, 590nm, 605nm, 625nm, 740nm.

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6. A set of five filters, transmission 370, 399, 450, 500, and 550 nm, for blocking Rayleigh lines and removal of the second order.